Current Issue : July - September Volume : 2013 Issue Number : 3 Articles : 7 Articles
The present study was carried out using the four seaweeds, Sargassum wightii, Padina tetrastromatica (Phaeophyceae) , Ulva faciata (chlorophyceae) and Gracilaria edulis (Rhodophyceae) which were found to be dominant in the areas of Arockiapuram, Kootapuli, Uvari , Manapad , Punnakayal from the south east coast of India. The seaweeds were analysed for the presence of important Phytochemical constituents. The effect of the different solvent extracts of the seaweeds were also analysed against eight bacterial pathogens isolated from different patient samples. The isolated pathogens were identified by biochemical tests and further confirmed by 16s rDNA sequencing. The study revealed that the antibacterial activity was significantly higher in methanolic extracts of the four dominant seaweeds and it was on par with the standard antibiotics....
The use of recent and advanced techniques of molecular biology for identifying bacteria are changing the way we practice clinical microbiology which affects the practice of medicine. Clinical microbiology laboratories have relied on phenotypic methods such as culture and biochemical tests for the detection, identification and characterization of virulence traits such as antibiotic resistance genes, toxins, etc of human pathogens. The advantages and disadvantages of molecular biology techniques including real-time Polymerase chain reaction (PCR), partial or whole genome sequencing, molecular typing, microarrays, broad-range PCR, direct hybridization, reverse hybridization and multiplexing over the traditional methods recommended by the British Pharmacopoeia will be addressed and discussed....
The wild Saccharomyces Cerevisiae consider as a good candidate for synthesizing high quantity of glutathione (red). GSH1 gene from Saccharomyces cerevisiae was identified and cloned in E.coli expression vector for over expression of glutathione synthetase leads to increase the levels of GSH in cellular extracts and the product purified to confirm its expression. Primers were designed based on entire sequence of Saccharomyces cerevisiae and GC content of the genomic DNA. The PCR carried out to amplify the template DNA with the primers. The amplified products were inserted in 1-67 & 99-346 region of PUC18 used as a cloning vector for the higher expression of glutathione production. In view of comparative analysis of glutathione preparation and standarized the protocol used in various steps involved in the purification process, subsequently identify the product recovered from different samples used in the experiment. The cellular glutathione content extracted in a supernatant of the culture lysate used for determined of glutathione. The efficiency of GSH production by recombinant E.coli has nearly 40 fold increased its production when compared to wild type strains. The prepared fractions were used for analysis of glutathione also the size of final purified products were checked by SDS PAGE and concentrated by lyophilization process, it is recommended that application of glutathione on protecting freeze dried molecules in various field....
The essential amino acid L-tryptophan (Trp) is used as a pharmaceutical agent, especially in neuro-medicine. It is also added into feed products as a food fortifier. Furthermore, application of Trp is widespread in biotechnology. Tryptophan is an amino acid necessary for growth in infants and for nitrogen balance in adults. Trp is produced by a condensation reaction between indole and L-serine, which is catalyzed by bacterial tryptophanase activity. In this review, we investigate Trp production using microbial system in the presence and absence of its precursors. In the present review on the production of tryptophan by using E.coli bacteria and many types, Fermentation condition and some application use in industries etc....
Protocol for micropropagation of Balanites aegyptiaca (L.) Del. using seeds was established. Seeds obtained from mature trees were sterilized and cultured into Murashige and Skoog (MS) medium. In-vitro raised seedlings nodal portion was used as explant. Explant culture were established in solid basal Murashige and skoog (MS) medium supplemented with different concentration and combination of kinetine puriss (6-furfuryl amino purine) 1, 3, 6 mg/l and indole-3-acetic acid (IAA) 0.1 mg/l for shoot proliferation and multiplication. The culture were maintained at 25±2°C under 7/5/12 hours/day i.e. 7 h/day photoperiod from cool white fluorescent lamps, 5 h/day dimlight, 12 h/day in dark. Result shows that shoot multiplication required 3 mg/l of kinetine puriss for sufficient growth....
organs under sterile conditions on a nutrient culture medium of known composition. Plant develoment is charactrized by the continuous initiation of tissues and organ. The meristem, which are small stem cell population, are involved in this process. The in vitro propagation of explants is influenced by cytokinin which makes its concentration adjustment necessary in order to optimize the multiplication rate . Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or used was consisted by macronutrients and micronutrients of MS medium. Nodal segment explants were excised from shoots cultured in MS medium without BAP, inoculated in flasks containing 30 ml of culture .Therefore, the plant cell and tissue require a proper medium for their growth and developmant . The nutrient media for plant cell is a well defined mixture of inorganic salts and typical carbon sources like sucrose and glucose. In majority of cell, supplementary constituents are required which are the essential growth regulators i. e. indole acetic acid (IAA), kinetin and NAA, vitamins like thiamin and nicotinic acid, amino acids, inositol and sugar alcohols....
Cellulose is one of the most abundantly polysaccharide found on the earth. Cellulose is the polymeric substance of the plant. Bacteria and fungi are microorganisms, known to produce extracellular cellulase when they grow on cellulosic materials under natural environment. The complete enzymatic hydrolysis of cellulosic material needs different types of cellulases such as endoglucanase exocellobio hydrolase and a-glucosidase.In the present review focused on the production of cellulase enzyme by using bacteria which isolated from garbage. This bacteriolytic cellulase enzyme will be useful in many industrial applications due its high tensile strength and water holding capacity. Such type of bacterolitic enzyme would be more economic than yeast & mold . Cellulases production has attracted much interest because of the diversity of their industrial application. With increasing application of bio-based fuels and chemicals, the production cellulases are increases rapidly. To create a sustainable bioeconomy, it is in need to produced cellulase which will be cost-effective. Cellulases enzymes are used in food, brewery and wine, animal feed, textile and laundry, pulp and paper industries, as well as in agriculture and for research purposes. The present article is an overview of the biotechnological state-of-the-art for cellulases....
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